Expression pattern of protease activated receptors in lymphoid cells

Cell Immunol. 2014 Mar-Apr;288(1-2):47-52. doi: 10.1016/j.cellimm.2014.02.004. Epub 2014 Feb 28.

Abstract

Protease-activated receptors (PARs) are a subfamily of four G-protein-coupled receptors mediating multiple functions. PARs expression was studied in subpopulations of human lymphocytes. Our results indicate that natural killer cells expressed mRNA for PAR₁, PAR₂ and PAR₃, CD4+ T cells expressed PAR₁ and PAR₂, while γδ and CD8+ T cells only expressed PAR₁. PAR₄ was absent at mRNA level and B cells did not express any PAR. Analyses of the cell surface PARs expression by flow cytometry were consistent with the mRNA data and also between different donors. PAR₁ is the most abundant member of the PAR family present in lymphocytes.

Keywords: B cells; CD8+ T cells; FACS; Lymphocytes; Natural killer cells, γδ cells, CD4+ T cells; Protease-activated receptors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • B-Lymphocytes / cytology
  • B-Lymphocytes / metabolism*
  • CD4 Antigens / genetics
  • CD4 Antigens / immunology
  • CD4-Positive T-Lymphocytes / cytology
  • CD4-Positive T-Lymphocytes / metabolism*
  • CD8-Positive T-Lymphocytes / cytology
  • CD8-Positive T-Lymphocytes / metabolism*
  • Gene Expression
  • Gene Expression Profiling
  • Humans
  • Killer Cells, Natural / cytology
  • Killer Cells, Natural / metabolism*
  • RNA, Messenger / genetics
  • RNA, Messenger / immunology
  • Receptor, PAR-1 / genetics*
  • Receptor, PAR-1 / immunology
  • Receptor, PAR-2 / genetics*
  • Receptor, PAR-2 / immunology
  • Receptors, Antigen, T-Cell, gamma-delta / genetics
  • Receptors, Antigen, T-Cell, gamma-delta / immunology
  • Receptors, Thrombin / genetics*
  • Receptors, Thrombin / immunology

Substances

  • CD4 Antigens
  • RNA, Messenger
  • Receptor, PAR-1
  • Receptor, PAR-2
  • Receptors, Antigen, T-Cell, gamma-delta
  • Receptors, Thrombin
  • protease-activated receptor 4