Abstract
Protease-activated receptors (PARs) are a subfamily of four G-protein-coupled receptors mediating multiple functions. PARs expression was studied in subpopulations of human lymphocytes. Our results indicate that natural killer cells expressed mRNA for PAR₁, PAR₂ and PAR₃, CD4+ T cells expressed PAR₁ and PAR₂, while γδ and CD8+ T cells only expressed PAR₁. PAR₄ was absent at mRNA level and B cells did not express any PAR. Analyses of the cell surface PARs expression by flow cytometry were consistent with the mRNA data and also between different donors. PAR₁ is the most abundant member of the PAR family present in lymphocytes.
Keywords:
B cells; CD8+ T cells; FACS; Lymphocytes; Natural killer cells, γδ cells, CD4+ T cells; Protease-activated receptors.
Copyright © 2014 Elsevier Inc. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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B-Lymphocytes / cytology
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B-Lymphocytes / metabolism*
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CD4 Antigens / genetics
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CD4 Antigens / immunology
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CD4-Positive T-Lymphocytes / cytology
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CD4-Positive T-Lymphocytes / metabolism*
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CD8-Positive T-Lymphocytes / cytology
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CD8-Positive T-Lymphocytes / metabolism*
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Gene Expression
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Gene Expression Profiling
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Humans
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Killer Cells, Natural / cytology
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Killer Cells, Natural / metabolism*
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RNA, Messenger / genetics
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RNA, Messenger / immunology
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Receptor, PAR-1 / genetics*
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Receptor, PAR-1 / immunology
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Receptor, PAR-2 / genetics*
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Receptor, PAR-2 / immunology
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Receptors, Antigen, T-Cell, gamma-delta / genetics
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Receptors, Antigen, T-Cell, gamma-delta / immunology
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Receptors, Thrombin / genetics*
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Receptors, Thrombin / immunology
Substances
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CD4 Antigens
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RNA, Messenger
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Receptor, PAR-1
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Receptor, PAR-2
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Receptors, Antigen, T-Cell, gamma-delta
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Receptors, Thrombin
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protease-activated receptor 4