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Microbes Infect. 2014 Jun;16(6):472-80. doi: 10.1016/j.micinf.2014.02.010. Epub 2014 Mar 14.

Comparison of human saliva and synthetic histo-blood group antigens usage as ligands in norovirus-like particle binding and blocking assays.

Author information

  • 1Vaccine Research Center, University of Tampere Medical School, Biokatu 10, FI-33520 Tampere, Finland.
  • 2Vaccine Research Center, University of Tampere Medical School, Biokatu 10, FI-33520 Tampere, Finland; Department of Pediatrics, Tampere University Hospital, Teiskontie 35, FI-33521 Tampere, Finland.
  • 3Vaccine Research Center, University of Tampere Medical School, Biokatu 10, FI-33520 Tampere, Finland. Electronic address: vesna.blazevic@uta.fi.

Abstract

Blocking of norovirus-like particle binding to their cellular ligands, histo-blood group antigens with immune sera, is considered a surrogate norovirus neutralization assay. We compared human secretor positive saliva and synthetic biotinylated carbohydrates as a source of histo-blood group antigens in binding and blocking assays. Six norovirus capsid-derived virus-like particles belonging to genogroup I (GI-1-2001 and GI-3-2002) and genogroup II (GII-4-1999, GII-4-2010 New Orleans, GII-4-2012 Sydney and GII-12-1998) noroviruses were produced by a recombinant baculovirus expression system and binding profile to saliva type A, B and O and to synthetic antigens (A trimer, B trimer, H type 1, H type 3, Lewis(a) and Lewis(b)) was identified. Good correlation between virus-like particle binding to saliva type A and synthetic A trimer (r = 0.66, p < 0.05) and saliva type B and synthetic B trimer (r = 0.75, p < 0.05) was observed. Binding of each norovirus virus-like particle to the selected histo-blood group antigens was blocked by convalescent sera from NoV-infected subjects or type-specific mouse antisera. Our results support the use of either saliva or synthetic antigens in blocking assay to measure the ability of norovirus antisera to block virus-like particle binding to the carbohydrate ligands.

Copyright © 2014 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

KEYWORDS:

Ligand binding; Neutralization; Norovirus; Virus-like particle

PMID:
24631874
[PubMed - indexed for MEDLINE]
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