Format

Send to

Choose Destination
See comment in PubMed Commons below
Anim Genet. 2014 Jun;45(3):445-8. doi: 10.1111/age.12136. Epub 2014 Mar 15.

KIT and melanoma predisposition in pigs: sequence variants and association analysis.

Author information

  • 1Departamento de Mejora Genética Animal, INIA, ctra de la Coruña Km7, 28040, Madrid, Spain.

Abstract

KIT mutations have been detected in different cancer subtypes, including melanoma. The gene also has been extensively studied in farm animals for its prominent role in coat color. The present work aimed at detecting KIT variants in a porcine model of cutaneous melanoma, the melanoblastoma-bearing Libechov Minipig (MeLiM). By sequencing exons and intron borders, 36 SNPs and one indel were identified. Of 10 coding SNPs, three were non-synonymous mutations, likely to affect the protein conformation. A promising variant, located in exon 19 (p.Val870Ala), was genotyped in a MeLiM × Duroc cross, and an association analysis was conducted on several melanoma-related traits. This variant showed a significant association with melanoma development, tumor ulceration and cutaneous invasion. In conclusion, although the KIT gene would not be a major causal gene for melanoma development in pig, its genetic variation could be influencing this trait.

© 2014 Stichting International Foundation for Animal Genetics.

KEYWORDS:

SNP; c-KIT; cancer; melanoblastoma-bearing Libechov Minipig; swine

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Wiley
    Loading ...
    Write to the Help Desk