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J Immunoassay Immunochem. 2015;36(1):100-10. doi: 10.1080/15321819.2014.899257.

The usage of phage mini-antibodies as a means of detecting ferritin concentration in animal blood serum.

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  • 1a Saratov Agricultural University of N.I. Vavilov , Saratov , Russia.

Abstract

Mini-antibodies that have specific ferritin response have been produced for the first time using sheep's phage libraries (Griffin.1, Medical Research Council, Cambridge, UK). Produced phage antibodies were used for the first time for the development of diagnostic test kits for ferritin detection in the blood of cattle. The immunodot assay with secondary biospecific labeling is suggested as means of ferritin detection in cow blood serum (antiferritin phage antibodies and rabbit antiphage antibodies conjugated with different labels). –°olloidal gold, gold nanoshells, and horse reddish peroxidase used as labels have shown a similar response while detecting concentration of ferritin (0.2 mg/mL). It is shown that the method of solid-phase immunoassay with a visual view of the results allows determination of the minimum concentration of ferritin in the blood of cows at 0.225 g/mL.

KEYWORDS:

colloidal gold; ferritin; immunodot; nanoshells; phage libraries

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