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1: Proc Natl Acad Sci U S A. 1988 Dec;85(24):9704-8.Click here to read Links

Identification, cloning, and characterization of an immune activation gene.

Lipes MA, Napolitano M, Jeang KT, Chang NT, Leonard WJ.

Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Bethesda, MD 20892.

We have identified an immune activation gene, denoted Act-2, by differential hybridization screening of an activated T-cell library. The gene is induced rapidly after T-cell activation with phytohemagglutinin, B-cell activation with Staphylococcus aureus Cowan I, and monocyte activation with lipopolysaccharide. We have isolated a cDNA containing the full-length coding region. The deduced amino acid sequence predicts an open reading frame of 92 amino acids, including a very hydrophobic N terminus, which by weight matrix score is predicted to be a signal peptide. Using a baculovirus expression system, we have shown that this gene encodes a secreted product. It is therefore possible that Act-2 represents a newly discovered cytokine.

PMID: 2462251 [PubMed - indexed for MEDLINE]

PMCID: PMC282843