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Sci Rep. 2014 Mar 12;4:4361. doi: 10.1038/srep04361.

Partial somatic to stem cell transformations induced by cell-permeable reprogramming factors.

Author information

  • 11] ProCell R&D Institute, ProCell Therapeutics, Inc., Ace-Twin Tower II, Guro3-dong, Guro-gu, Seoul 152-790, Korea [2].
  • 2ProCell R&D Institute, ProCell Therapeutics, Inc., Ace-Twin Tower II, Guro3-dong, Guro-gu, Seoul 152-790, Korea.
  • 31] ProCell R&D Institute, ProCell Therapeutics, Inc., Ace-Twin Tower II, Guro3-dong, Guro-gu, Seoul 152-790, Korea [2] Department of Biomedical Sciences, Chonnam National University Medical School, 5 Hak-dong, Dong-gu, Kwangju 501-757, Korea [3] Departments of Surgery, Vanderbilt University School of Medicine, 2215B Garland Avenue, Nashville, TN 37232, USA.

Abstract

The production of pluripotent stem cells (iPSCs) for therapeutic applications will require practical methods to achieve tight temporal and quantitative control of reprogramming factor (RF) expression, while avoiding the mutagenic potential of gene transfer. Toward this end, we have developed cell-permeable RF proteins (CP-RFs) incorporating newly developed macromolecule transduction domains (MTDs). Treatment of human dermal fibroblasts (HDFs) with combinations of cell-permeable OCT4, SOX2, KLF4, CMYC and either NANOG or LIN28 proteins induced the outgrowth of stem cell-like colonies (iSCs). iSC colonies generated with CP-RFs resembled embryonic stem cells with regard to morphology, biomarker expression, and extended capacity for self-renewal, but failed to expand as iPSC or ES cell lines. Partial reprogramming appears to be a common response to protein-based delivery of programming factors into somatic cells.

PMID:
24618595
[PubMed - indexed for MEDLINE]
PMCID:
PMC3950638
Free PMC Article
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