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Regen Med. 2014;9(5):593-607. doi: 10.2217/rme.14.17. Epub 2014 Mar 12.

Multipotential stromal cell abundance in cellular bone allograft: comparison with fresh age-matched iliac crest bone and bone marrow aspirate.

Author information

  • 1Leeds Institute of Rheumatic & Musculoskeletal Medicine, Room 5.24 Clinical Sciences Building, University of Leeds, Leeds, LS9 7TF, UK. msjej@leeds.ac.uk.

Abstract

AIM:

To enumerate and characterize multipotential stromal cells (MSCs) in a cellular bone allograft and compare with fresh age-matched iliac crest bone and bone marrow (BM) aspirate.

MATERIALS & METHODS:

MSC characterization used functional assays, confocal/scanning electron microscopy and whole-genome microarrays. Resident MSCs were enumerated by flow cytometry following enzymatic extraction.

RESULTS:

Allograft material contained live osteocytes and proliferative bone-lining cells defined as MSCs by phenotypic and functional capacities. Without cultivation/expansion, the allograft displayed an 'osteoinductive' molecular signature and the presence of CD45(-)CD271(+)CD73(+)CD90(+)CD105(+) MSCs; with a purity over 100-fold that of iliac crest bone. In comparison with BM, MSC numbers enzymatically released from 1 g of cellular allograft were equivalent to approximately 45 ml of BM aspirate.

CONCLUSION:

Cellular allograft bone represents a unique nonimmune material rich in MSCs and osteocytes. This osteoinductive graft represents an attractive alternative to autograft bone or composite/synthetic grafts in orthopedics and broader regenerative medicine settings.

KEYWORDS:

bone marrow; cellular allograft; multipotential stromal cells; osteocytes

PMID:
24617969
[PubMed - indexed for MEDLINE]
PMCID:
PMC4077757
Free PMC Article
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