Abstract
The receptor for bombesin and the related peptide, gastrin-releasing peptide (GRP) has been induced in frog oocytes by injection of polyA+ RNA from rat brain. The primed oocytes responded to peptides of the bombesin family (GRP, neuromedin C of bombesin) by showing dose-dependent oscillations in membrane currents as recorded by the voltage-clamp method. The induced membrane changes were suppressed when oocytes were pretreated with a bombesin-receptor antagonist.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bombesin / metabolism*
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Brain / metabolism*
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Cell Membrane / physiology
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Female
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Gastrin-Releasing Peptide
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Membrane Potentials
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Microinjections
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Oocytes / physiology*
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Peptides / metabolism*
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Poly A / genetics*
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Protein Biosynthesis
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RNA / genetics*
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RNA, Messenger
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Rats
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Receptors, Bombesin
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Receptors, Neurotransmitter / genetics*
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Receptors, Neurotransmitter / physiology
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Xenopus laevis
Substances
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Peptides
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RNA, Messenger
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Receptors, Bombesin
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Receptors, Neurotransmitter
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Poly A
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RNA
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Gastrin-Releasing Peptide
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Bombesin