Expression and electrophysiological identification of the receptor for bombesin and gastrin-releasing peptide in Xenopus laevis oocytes injected with polyA+ RNA from rat brain

FEBS Lett. 1988 Oct 24;239(1):109-12. doi: 10.1016/0014-5793(88)80555-6.

Abstract

The receptor for bombesin and the related peptide, gastrin-releasing peptide (GRP) has been induced in frog oocytes by injection of polyA+ RNA from rat brain. The primed oocytes responded to peptides of the bombesin family (GRP, neuromedin C of bombesin) by showing dose-dependent oscillations in membrane currents as recorded by the voltage-clamp method. The induced membrane changes were suppressed when oocytes were pretreated with a bombesin-receptor antagonist.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bombesin / metabolism*
  • Brain / metabolism*
  • Cell Membrane / physiology
  • Female
  • Gastrin-Releasing Peptide
  • Membrane Potentials
  • Microinjections
  • Oocytes / physiology*
  • Peptides / metabolism*
  • Poly A / genetics*
  • Protein Biosynthesis
  • RNA / genetics*
  • RNA, Messenger
  • Rats
  • Receptors, Bombesin
  • Receptors, Neurotransmitter / genetics*
  • Receptors, Neurotransmitter / physiology
  • Xenopus laevis

Substances

  • Peptides
  • RNA, Messenger
  • Receptors, Bombesin
  • Receptors, Neurotransmitter
  • Poly A
  • RNA
  • Gastrin-Releasing Peptide
  • Bombesin