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Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2014 Feb;22(1):99-103. doi: 10.7534/j.issn.1009-2137.2014.01.020.

[Expression of miR-16 in patients with T lymphoblastic lymphoma/acute lymphoblastic leukemia].

[Article in Chinese]

Author information

  • 1Department of Hematology, Yixing Municipal People's Hospital, Yixing, 214200, Jiangsu Province, China.
  • 2Department of Hematology, Yixing Municipal People's Hospital, Yixing, 214200, Jiangsu Province, China. E-mail: staff324@yxph.com.

Abstract

This study was purposed to investigate the expression of miR-16 in T lymphoblastic lymphoma/acute lymphoblastic leukemia (T-LBL/ALL) and its relation with target therapy and prognosis. The CD3, cCD3, CD10, CD20, CD34, CD43, CD99, TdT, PAX-5, BCL-2 and Ki67 in paraffin samples from 38 cases of T-LBL/ALL were detected by immunohistochemical labeling; the miR-16 expression level was detected by real-time RT-PCR. Fifteen cases of reactive hyperplasia of lymph nodes were selected as control. The results indicated that among 38 cases of T-LBL/ALL the positive rate of TdT was highest (94.7%), the positive rate of CD34 was lowest (22.1%), the PAX-5 and CD20 were found to be negative. The Ki67 expression level in 39.5% cases exceeded 80%. As compared with reactive hyperplasia of lymph node, the miR-16 expression in T-LBL/ALL was up-regulated, ant its expression level was 4.87-fold of reactive hyperplasia of lymph node (P < 0.05). The overall survival rate in group of miR-16 high expression decreased (P < 0.05). The prognosis of T-LBL/ALL patients with BCL-2 positive expression was better than that of patients with BCL-2 negative expression (P < 0.05). The miR-16 expression correlated with BCL-2 protein (r = 0.51, P < 0.05). It is concluded that the overall survival rate in miR-16 high expression group is higher than that in miR-16 low expression group, suggesting possible relation of miR-16 with prognosis. Moreover, the prognosis in BCL-2 positive expression group is better than that in negative expression group, which may be a factor influencing prognosis.

PMID:
24598659
[PubMed - indexed for MEDLINE]
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