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J Sep Sci. 2014 May;37(9-10):1141-7. doi: 10.1002/jssc.201301159. Epub 2014 Apr 10.

Comparison of reversed-phase liquid chromatography and hydrophilic interaction chromatography for the fingerprint analysis of Radix isatidis.

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  • 1College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China; Military Institute of Chinese Materia Medica, 302th Military Hospital of China, Beijing, China.


Radix isatidis is a famous anti-influenza virus herbal medicine traditionally taken as a water decoction. However, the chemical fingerprint analysis of Radix isatidis is dominantly based on RPLC, from which it is difficult to obtain fingerprint information of hydrophilic compounds. Here, we developed the separation of Radix isatidis by RPLC and hydrophilic interaction chromatography, comparing the traditional RPLC fingerprint with the hydrophilic interaction chromatography fingerprint. Besides, an anti-viral assay of Radix isatidis was conducted to evaluate its efficacy. The fingerprint-efficacy relationships between the fingerprints and the anti-viral activity were further investigated with principal component regression analysis. The results showed that the anti-viral activity correlated better with the hydrophilic interaction chromatography fingerprint than with the RPLC fingerprint. This study indicates that hydrophilic interaction chromatography could not only be a complementary method to increase the fingerprint coverage of conventional RPLC fingerprint, but also can better represent the efficacy and quality of Radix isatidis.

© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Fingerprint analysis; Hydrophilic interaction chromatography; Radix isatidis; Reversed-phase liquid chromatography

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