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Methods Mol Biol. 2014;1140:107-16. doi: 10.1007/978-1-4939-0354-2_8.

Eukaryotic expression systems for structural studies.

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  • 1Center for Structural Genomics of Infectious Diseases, Computational Institute, University of Chicago, Chicago, IL, 60557, USA.

Abstract

This protocol describes protein production in mammalian cells by transient transfection. It assumes the expression construct contains either a 6-HIS or Fc fusion tag to allow recovery of the protein by affinity chromatography. The method is one of the simplest available for protein expression in eukaryotic cells, requires little specialized equipment, and has a reasonably high rate of success.

PMID:
24590712
[PubMed - indexed for MEDLINE]
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