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Viruses. 2014 Feb 28;6(3):1004-14. doi: 10.3390/v6031004.

Isolation and identification of feline herpesvirus type 1 from a South China tiger in China.

Author information

  • 1College of Wildlife Resource, Northeast Forestry University, Haerbin, 150040, China. xiaofengsht@163.com.
  • 2College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, 730070, China. liyuanguo0520@163.com.
  • 3Wildlife Rescue and Breeding Centre of Jilin Province, Changchun, 130122, China. jiaoweiyi2013@163.com.
  • 4Wildlife Rescue and Breeding Centre of Jilin Province, Changchun, 130122, China. liucunfa2013@163.com.
  • 5Wildlife Rescue and Breeding Centre of Jilin Province, Changchun, 130122, China. liuxiujuan2013@126.com.
  • 6Wildlife Rescue and Breeding Centre of Jilin Province, Changchun, 130122, China. haijunwang2013@163.com.
  • 7Shenzhen Wildlife Zoo, Shenzhen, 518055, China. huafuyou2013@163.com.
  • 8Shenzhen Wildlife Zoo, Shenzhen, 518055, China. dongjianxiu2013@163.com.
  • 9Military Veterinary Research Institute of Academy of Military Medical Sciences, Changchun, 130122, China. fst0007@163.com.
  • 10Military Veterinary Research Institute of Academy of Military Medical Sciences, Changchun, 130122, China. zhijun0215@gmail.com.
  • 11Military Veterinary Research Institute of Academy of Military Medical Sciences, Changchun, 130122, China. gaoyuwei@gmail.com.
  • 12Military Veterinary Research Institute of Academy of Military Medical Sciences, Changchun, 130122, China. gywtext@hotmail.com.

Abstract

In 2012, an FHV-1-like virus was isolated from a tiger that presented with clinical signs of sialorrhea, sneezing and purulent rhinorrhea. Isolation was performed with the FK81 cell line, and the virus was identified by PCR, transmission electron microscopy (TEM), and the phylogenetic analysis of the partial thymidine kinase (TK) and glycoprotein B (gB) genes. A total of 253 bp of the TK gene and 566 bp of the gB gene were amplified from the trachea of the tiger by PCR/RT-PCR method. Phylogenetic analysis showed that the isolate belonged to the same cluster with other FHV-1 strains obtained from GenBank. Herpes-like viruses with an envelope and diameters of approximately 200 nm were observed in the culture supernatants of FK81 cells inoculated with samples from the tiger. The FHV-1 infection was confirmed by an animal challenge experiment in a cat model. Our finding extends the host range of FHV-1 and has implications for FHV-1 infection and South China tiger conservation.

PMID:
24590411
[PubMed - indexed for MEDLINE]
PMCID:
PMC3970135
Free PMC Article
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