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Acta Biomater. 2014 Jun;10(6):2455-62. doi: 10.1016/j.actbio.2014.02.038. Epub 2014 Feb 28.

Surface topography and chemistry shape cellular behavior on wide band-gap semiconductors.

Author information

  • 1UNC/NCSU Joint Department of Biomedical Engineering, North Carolina State University, Raleigh, NC 27606, USA.
  • 2Department of Materials Science and Engineering, North Carolina State University, Raleigh, NC 27606, USA.
  • 3Birck Nanotechnology Center, School of Materials Engineering, Purdue University, West Lafayette, IN 47907, USA.
  • 4Department of Physics, Purdue University, West Lafayette, IN 47907, USA.
  • 5Birck Nanotechnology Center, School of Materials Engineering, Purdue University, West Lafayette, IN 47907, USA; Department of Physics, Purdue University, West Lafayette, IN 47907, USA; School of Electrical and Computer Engineering, Purdue University, West Lafayette, IN 47907, USA.
  • 6UNC/NCSU Joint Department of Biomedical Engineering, North Carolina State University, Raleigh, NC 27606, USA; Department of Materials Science and Engineering, North Carolina State University, Raleigh, NC 27606, USA. Electronic address: Ivanisevic@ncsu.edu.

Abstract

The chemical stability and electrical properties of gallium nitride make it a promising material for the development of biocompatible electronics, a range of devices including biosensors as well as interfaces for probing and controlling cellular growth and signaling. To improve the interface formed between the probe material and the cell or biosystem, surface topography and chemistry can be applied to modify the ways in which the device interacts with its environment. PC12 cells are cultured on as-grown planar, unidirectionally polished, etched nanoporous and nanowire GaN surfaces with and without a physisorbed peptide sequence that promotes cell adhesion. While cells demonstrate preferential adhesion to roughened surfaces over as-grown flat surfaces, the topography of that roughness also influences the morphology of cellular adhesion and differentiation in neurotypic cells. Addition of the peptide sequence generally contributes further to cellular adhesion and promotes development of stereotypic long, thin neurite outgrowths over alternate morphologies. The dependence of cell behavior on both the topographic morphology and surface chemistry is thus demonstrated, providing further evidence for the importance of surface modification for modulating bio-inorganic interfaces.

Copyright © 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

KEYWORDS:

Cell differentiation; Gallium nitride; Rat pheochromocytoma (PC12) cells; Surface chemistry; Surface topography

[PubMed - indexed for MEDLINE]
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