Format

Send to:

Choose Destination
See comment in PubMed Commons below
Clin Chim Acta. 2014 Apr 20;431:288-93. doi: 10.1016/j.cca.2014.02.018. Epub 2014 Feb 28.

LDL cholesterol performance of beta quantification reference measurement procedure.

Author information

  • 1National Cerebral and Cardiovascular Center, Department of Preventive Cardiology, Lipid Reference Laboratory, Japan. Electronic address: nakamura.masakazu.hp@ncvc.go.jp.
  • 2Department of Health Sciences, Faculty of Medical Sciences, Kyushu University, Japan.
  • 3Public Health, Department of Social and Environmental Medicine, Graduate School of Medicine, Osaka University, Japan.
  • 4Osaka Center for Cancer and Cardiovascular Disease Prevention, Japan.
  • 5National Cerebral and Cardiovascular Center, Department of Preventive Cardiology, Lipid Reference Laboratory, Japan.
  • 6National Cerebral and Cardiovascular Center, Department of Preventive Medicine and Epidemiologic Informatics, Office of Evidence-based Medicine and Risk Analysis, Japan.
  • 7National Cerebral and Cardiovascular Center, Department of Preventive Medicine and Epidemiologic Informatics, Japan.
  • 8Public Health, Department of Social and Environmental Medicine, Graduate School of Medicine, Osaka University, Japan; Ministry of Health, Labour and Welfare, Health Service Bureau, Cancer Control and Health Promotion Division, Japan.
  • 9Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, USA.
  • 10National Cerebral and Cardiovascular Center, Department of Preventive Cardiology, Department of Preventive Medicine and Epidemiologic Informatics, Japan.

Abstract

BACKGROUND:

Accurate measurement of blood lipids is crucial in cardiovascular disease risk management. The Centers for Disease Control and Prevention (CDC) Cholesterol Reference Method Laboratory Network (CRMLN) has assured the accuracy of these measurements for over 20 years using beta quantification (BQ) method as reference measurement procedure (RMP) for high- and low-density lipoprotein cholesterol (HDL-C, LDL-C). Only limited data exist about the performance of the BQ RMP.

METHODS:

Bottom fraction cholesterol (BFC), HDL-C, and LDL-C results after ultracentrifugation from the CDC lipid reference laboratory and the Japanese CRMLN laboratory were compared using 280 serum samples measured over the past 15 years. Data were compared statistically using method comparison and bias estimation analysis.

RESULTS:

Regression analysis between CDC (x) and Osaka (y) for BFC, HDL-C, and LDL-C were y=0.988x+1.794 (R(2)=0.997), y=0.980x+1.118 (R(2)=0.994), and y=0.987x+1.200 (R(2)=0.997), respectively. The Osaka laboratory met performance goals for 90% to 95% of the CDC reference values.

CONCLUSIONS:

The BQ method by the Osaka CRMLN laboratory is highly accurate and has been stable for over 15 years. Accurate measurement of BFC is critical for the determination of LDL-C.

Copyright © 2014 Elsevier B.V. All rights reserved.

KEYWORDS:

Beta quantification; Bottom fraction cholesterol; HDL cholesterol; LDL cholesterol

PMID:
24589772
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk