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PLoS One. 2014 Feb 20;9(2):e89387. doi: 10.1371/journal.pone.0089387. eCollection 2014.

βν integrin inhibits chronic and high level activation of JNK to repress senescence phenotypes in Drosophila adult midgut.

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  • 1Department of Life Science, Faculty of Science, Gakushuin University, Tokyo, Japan.
  • 2Department of Life Science, Faculty of Science, Gakushuin University, Tokyo, Japan ; Graduate Course in Life Science, Graduate School of Science, Gakushuin University, Tokyo, Japan.
  • 3Department of Life Science, Faculty of Science, Gakushuin University, Tokyo, Japan ; Graduate Course in Life Science, Graduate School of Science, Gakushuin University, Tokyo, Japan ; Institute for Biomolecular Science, Gakushuin University, Tokyo, Japan.

Abstract

Proper control of adult stem cells including their proliferation and differentiation is crucial in maintaining homeostasis of well-organized tissues/organs throughout an organism's life. The Drosophila adult midgut has intestinal stem cells (ISCs), which have been exploited as a simple model system to investigate mechanisms controlling adult tissue homeostasis. Here, we found that a viable mutant of βν integrin (βint-ν), encoding one of two Drosophila integrin β subunits, showed a short midgut and abnormal multilayered epithelia accompanied by an increase in ISC proliferation and misdifferentiation defects. The increase in ISC proliferation and misdifferentiation was due to frequent ISC duplication expanding a pool of ISCs, which was caused by depression of the Notch signalling, and up-regulation of unpaired (upd), a gene encoding an extracellular ligand in the JAK/STAT signalling pathway. In addition, we observed that abnormally high accumulation of filamentous actin (F-actin) was caused in the βint-ν mutant enterocytes. Furthermore, the defects were rescued by suppressing c-Jun N-terminal kinase (JNK) signalling, which was up-regulated in a manner correlated with the defect levels in the above-mentioned βint-ν mutant phenotype. These symptoms observed in young βint-ν mutant midgut were very similar to those in the aged midgut in wild type. Our results suggested that βint-ν has a novel function for the Drosophila adult midgut homeostasis under normal conditions and provided a new insight into possible age-related diseases caused by latent abnormality of an integrin function.

PMID:
24586740
[PubMed - indexed for MEDLINE]
PMCID:
PMC3930726
Free PMC Article
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