Format

Send to

Choose Destination
See comment in PubMed Commons below
Antiviral Res. 2014 May;105:47-53. doi: 10.1016/j.antiviral.2014.02.018. Epub 2014 Feb 28.

Determining anti-betanodavirus compounds through a GF-1 cell-based screening platform.

Author information

  • 1Institute of Fisheries Science, College of Life Science, National Taiwan University, Taipei, Taiwan.
  • 2Institute of Fisheries Science, College of Life Science, National Taiwan University, Taipei, Taiwan; Department of Life Science, College of Life Science, National Taiwan University, Taipei, Taiwan. Electronic address: yshan@ntu.edu.tw.

Abstract

Betanodavirus is a highly contagious pathogen, responsible for severe losses incurred in the aquaculture industry. Currently, there are no commercially available antivirals against the virulence observed during very early stages of fish larvae development. Therefore, we developed a novel GF-1 (grouper fin cell) cell viability-based screening assay to facilitate the discovery of an anti-betanodavirus agent. The assay conditions were optimized and the robustness of the assay was confirmed by a Z' factor value ranging from 0.7 to 0.94. After screening a library of 2000 small molecule compounds, 43 compounds with a virus inhibition capacity of ⩾55% were identified. A cytochrome P450 inhibitor, proadifen hydrochloride, was validated with an EC50 value of 6.48μM and a CC50 value of 20.63μM. This compound inhibited the amplification of viral RNA by 99.68% 5days post-infection. Surprisingly, we found that 18 of 43 compounds act as neurotransmitter agents. These findings indicate a novel way of investigating the infection mechanism of betanodavirus, and suggest potential candidates for an anti-betanodavirus drug.

Copyright © 2014 Elsevier B.V. All rights reserved.

KEYWORDS:

Antiviral drug screening; Betanodavirus; Grouper culture; Nervous necrosis virus

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk