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Vet Pathol. 2015 Jan;52(1):92-6. doi: 10.1177/0300985814522817. Epub 2014 Feb 24.

Cellular sources of tenascin-C in canine mammary carcinomas.

Author information

  • 1Department of Veterinary Pathology, Nippon Veterinary and Life Science University, Tokyo, Japan Departments of Pathology and Integrative Oncological Pathology, Nippon Medical School, Tokyo, Japan.
  • 2Department of Veterinary Pathology, Nippon Veterinary and Life Science University, Tokyo, Japan.
  • 3Departments of Pathology and Integrative Oncological Pathology, Nippon Medical School, Tokyo, Japan.
  • 4Department of Veterinary Pathology, Nippon Veterinary and Life Science University, Tokyo, Japan kimimasa@nvlu.ac.jp.

Abstract

Tenascin-C (Tn-C) is an extracellular matrix glycoprotein implicated in the progression of several human cancers. In canine mammary carcinomas, accumulation of Tn-C has been recognized in 3 different areas: regions of proliferating myoepithelial cells in complex carcinoma, basement membrane zone in low-grade simple carcinoma, and reactive stroma in high-grade simple carcinoma. To identify the Tn-C synthesizing cells in these areas, we utilized double-labeling immunohistochemistry, branched DNA in situ hybridization, and in situ hybridization-immunohistochemistry double-labeling techniques. In complex carcinomas, Tn-C was generated by proliferating myoepithelial cells. Tn-C in low-grade simple carcinomas was also derived from myoepithelial cells existing as a basal monolayer. However, stromal Tn-C in high-grade carcinomas was mainly synthesized by fibroblasts/myofibroblasts, similar to human breast cancer. Thus, the origin of Tn-C in canine mammary carcinomas differs between low- and high-grade malignancies. The role of myoepithelial cell-generated Tn-C is not yet understood.

© The Author(s) 2014.

KEYWORDS:

dogs; immunohistochemistry; in situ hybridization; mammary carcinoma; mammary tumor; myoepithelial cell; myofibroblast; tenascin-C

PMID:
24565830
[PubMed - in process]
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