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Res Vet Sci. 2014 Apr;96(2):276-82. doi: 10.1016/j.rvsc.2014.01.005. Epub 2014 Feb 11.

Antiproliferative effect of Canavalia brasiliensis lectin on B16F10 cells.

Author information

  • 1Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, Pernambuco, Brazil. Electronic address: foliveirasilva@gmail.com.
  • 2Centro de Ciências biológicas, Universidade Federal de Pernambuco, Pernambuco, Brazil.
  • 3Departamento de Imunologia do Centro de Pesquisas Aggeu Magalhães - CPqAM/FIOCRUZ-UFPE, Pernambuco, Brazil.
  • 4Biomol-Lab, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Brazil.
  • 5Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Brazil.
  • 6Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, Pernambuco, Brazil. Electronic address: analuporto@yahoo.com.br.

Abstract

Lectins are proteins or glycoproteins with the ability to link to carbohydrates at the cell surface in a specific and reversible manner. Studies have showed that lectins are demonstrate immunomodulatory and antitumor agents. This study aims to evaluate the effect of lectin extracted from the seeds of Canavalia brasiliensis (ConBr) on murine melanoma B16F10 cells by analyzing cell viability, apoptosis index, cell migration, production of cytokines and nitric oxide (NO). Results showed that ConBr was able to reduce cell viability and thwart apoptosis, which could be observed by decrease in cell migration. ConBr also induced NO and IL-12 synthesis. Altogether, these data demonstrate the potential of ConBr as a therapeutic agent for melanoma.

Copyright © 2014 Elsevier Ltd. All rights reserved.

KEYWORDS:

Antiproliferative activity; Canavalia brasiliensis; ConBr; Glucose/manose binding lectin; Murine melanoma

PMID:
24565003
[PubMed - indexed for MEDLINE]
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