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Redox Biol. 2014 Feb 3;2:368-76. doi: 10.1016/j.redox.2014.01.019. eCollection 2014.

Optogenetic control of ROS production.

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  • 1Department of Medicine, University of Rochester Medical Center, Box 711, 601 Elmwood Avenue, Rochester, NY 14642, USA.
  • 2Department of Imaging Sciences, University of Rochester Medical Center, Rochester, NY, USA.


Reactive Oxygen Species (ROS) are known to cause oxidative damage to DNA, proteins and lipids. In addition, recent evidence suggests that ROS can also initiate signaling cascades that respond to stress and modify specific redox-sensitive moieties as a regulatory mechanism. This suggests that ROS are physiologically-relevant signaling molecules. However, these sensor/effector molecules are not uniformly distributed throughout the cell. Moreover, localized ROS damage may elicit site-specific compensatory measures. Thus, the impact of ROS can be likened to that of calcium, a ubiquitous second messenger, leading to the prediction that their effects are exquisitely dependent upon their location, quantity and even the timing of generation. Despite this prediction, ROS signaling is most commonly intuited through the global administration of chemicals that produce ROS or by ROS quenching through global application of antioxidants. Optogenetics, which uses light to control the activity of genetically-encoded effector proteins, provides a means of circumventing this limitation. Photo-inducible genetically-encoded ROS-generating proteins (RGPs) were originally employed for their phototoxic effects and cell ablation. However, reducing irradiance and/or fluence can achieve sub-lethal levels of ROS that may mediate subtle signaling effects. Hence, transgenic expression of RGPs as fusions to native proteins gives researchers a new tool to exert spatial and temporal control over ROS production. This review will focus on the new frontier defined by the experimental use of RGPs to study ROS signaling.


CALI, chromophore-assisted light inactivation; KR, KillerRed; KillerRed; Optogenetics; PDT, photodynamic therapy; Photodynamic therapy; Phototoxicity; RGP, ROS generating protein; ROS signaling; ROS, Reactive Oxygen Species; miniSOG; miniSOG, mini Singlet Oxygen Generator

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