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Se Pu. 2013 Nov;31(11):1035-9.

[Preparation of a new stationary phase with s-triazine and amide embedded and its application in separation of basic compounds].

[Article in Chinese]

Author information

  • 1Industrial Chemistry Institute, Nanjing University of Science and Technology, Nanjing 210094, China.
  • 2Dalian Elite Analytical Instruments Co., Ltd., Dalian 116023, China.

Abstract

A new kind of silica-bonded reserved stationary phase with s-triazine and amide polar groups embedded for HPLC was obtained by step-wise reactions of silica gel with s-triazine, gamma-aminopropyltriethoxysilane, ethanediamine and dodecanoyl chloride. The new stationary phase was characterized by elemental analysis. After that, a column packed with the new stationary phase was used to separate basic compounds. A commercial C18 column was also tested under the same chromatographic conditions for comparison. The results indicated that the ligand was successfully bonded to the surface of the silica and the maximum relative deviations of element contents were within 5% for carbon, nitrogen and hydrogen by three times. Furthermore, in the separation of five basic anilines and four basic pyridines, the excellent selectivity and symmetrical peak shapes provided a reference for advancing the commercialization of the new stationary phase.

PMID:
24558836
[PubMed]
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