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Biosens Bioelectron. 2014 Jul 15;57:59-64. doi: 10.1016/j.bios.2014.01.052. Epub 2014 Feb 3.

Photothermal spectral-domain optical coherence reflectometry for direct measurement of hemoglobin concentration of erythrocytes.

Author information

  • 1School of Mechanical Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-749, Republic of Korea.
  • 2Department of Laboratory Medicine, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-749, Republic of Korea.
  • 3School of Mechanical Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-749, Republic of Korea. Electronic address: cjoo@yonsei.ac.kr.

Abstract

A novel optical detection method for hemoglobin concentration is described. The hemoglobin molecules consisting mainly of iron generate heat upon their absorption of light energy at 532 nm, which subsequently changes the refractive index of the blood. We exploit this photothermal effect to determine the hemoglobin concentration of erythrocytes without any preprocessing of blood. Highly sensitive measurement of refractive index alteration of blood samples is enabled by a spectral-domain low coherence reflectometric sensor with subnanometer-level optical path-length sensitivity. The performance and validity of the sensor are presented by comparing the measured results against the reference data acquired from an automatic hematology analyzer.

Copyright © 2014 Elsevier B.V. All rights reserved.

KEYWORDS:

Erythrocytes; Hemoglobin concentration; Low coherence interferometry; Photothermal effect; Refractive index

[PubMed - indexed for MEDLINE]
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