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Acta Histochem. 2014 Jun;116(5):702-7. doi: 10.1016/j.acthis.2013.12.011. Epub 2014 Feb 12.

Immunohistochemical localization of D-serine dehydratase in chicken tissues.

Author information

  • 1Department of Orthopaedic Surgery, Shiga University of Medical Science, Seta, Ohtsu, Shiga 520-2192, Japan.
  • 2Department of Biochemistry and Molecular Biology, Shiga University of Medical Science, Seta, Ohtsu, Shiga 520-2192, Japan. Electronic address: tanakah@belle.shiga-med.ac.jp.
  • 3Department of Biochemistry and Molecular Biology, Shiga University of Medical Science, Seta, Ohtsu, Shiga 520-2192, Japan.

Abstract

Chicken D-serine dehydratase (DSD) degrades d-serine to pyruvate and ammonia. The enzyme requires both pyridoxal 5'-phosphate and Zn(2+) for its activity. d-Serine is a physiological coagonist that regulates the activity of the N-methyl-d-aspartate receptor (NMDAR) for l-glutamate. We have recently found in chickens that d-serine is degraded only by DSD in the brain, whereas it is also degraded to 3-hydroxypyruvate by d-amino acid oxidase (DAO) in the kidney and liver. In mammalian brains, d-serine is degraded only by DAO. It has not been clarified why chickens selectively use DSD for the control of d-serine concentrations in the brain. In the present study, we measured DSD activity in chicken tissues, and examined the cellular localization of DSD using a specific anti-chicken DSD antibody. The highest activity was found in kidney. Skeletal muscles and heart showed no activity. In chicken brain, cerebellum showed about 6-fold-higher activity (1.1 ± 0.3 U/g protein) than cerebrum (0.19 ± 0.03 U/g protein). At the cellular level DSD was demonstrated in proximal tubule cells of the kidney, in hepatocytes, in Bergmann-glia cells of the cerebellum and in astrocytes. The finding of DSD in glial cells seems to be important because d-serine is involved in NMDAR-dependent brain functions.

Copyright © 2014 Elsevier GmbH. All rights reserved.

KEYWORDS:

Astrocyte; Chicken brain; Immunohistochemistry; d-Serine; d-Serine dehydratase

PMID:
24529545
[PubMed - indexed for MEDLINE]
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