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Anal Chim Acta. 2014 Feb 27;813:41-7. doi: 10.1016/j.aca.2014.01.026. Epub 2014 Jan 16.

Simple diazonium chemistry to develop specific gene sensing platforms.

Author information

  • 1Department of Analytical Chemistry, Universidad Autónoma de Madrid, 28049 Madrid, Spain. Electronic address: monica.revenga@uam.es.
  • 2Department of Analytical Chemistry, Universidad Autónoma de Madrid, 28049 Madrid, Spain. Electronic address: tania.garcia@uam.es.
  • 3Department of Analytical and Food Chemistry, Universidad de Vigo, 36310 Vigo, Pontevedra, Spain. Electronic address: vichu@uvigo.es.
  • 4Department of Analytical and Food Chemistry, Universidad de Vigo, 36310 Vigo, Pontevedra, Spain. Electronic address: eromero@uvigo.es.
  • 5Department of Applied Physics, Universidad Autónoma de Madrid, 28049 Madrid, Spain. Electronic address: antonio.garciamarin@uam.es.
  • 6Department of Applied Physics, Universidad Autónoma de Madrid, 28049 Madrid, Spain. Electronic address: joseluis.pau@uam.es.
  • 7Department of Analytical Chemistry, Universidad Autónoma de Madrid, 28049 Madrid, Spain. Electronic address: felix.pariente@uam.es.
  • 8Department of Analytical Chemistry, Universidad Autónoma de Madrid, 28049 Madrid, Spain; IMDEA Nanociencia, Madrid, Spain. Electronic address: encarnacion.lorenzo@uam.es.

Abstract

A simple strategy for covalent immobilizing DNA sequences, based on the formation of stable diazonized conducting platforms, is described. The electrochemical reduction of 4-nitrobenzenediazonium salt onto screen-printed carbon electrodes (SPCE) in aqueous media gives rise to terminal grafted amino groups. The presence of primary aromatic amines allows the formation of diazonium cations capable to react with the amines present at the DNA capture probe. As a comparison a second strategy based on the binding of aminated DNA capture probes to the developed diazonized conducting platforms through a crosslinking agent was also employed. The resulting DNA sensing platforms were characterized by cyclic voltammetry, electrochemical impedance spectroscopy and spectroscopic ellipsometry. The hybridization event with the complementary sequence was detected using hexaamineruthenium (III) chloride as electrochemical indicator. Finally, they were applied to the analysis of a 145-bp sequence from the human gene MRP3, reaching a detection limit of 210 pg μL(-1).

Copyright © 2014 Elsevier B.V. All rights reserved.

KEYWORDS:

DNA sensing platform; Diazonium chemistry; Real DNA sample detection

[PubMed - indexed for MEDLINE]
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