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Anal Biochem. 2014 May 1;452:13-5. doi: 10.1016/j.ab.2014.02.001. Epub 2014 Feb 10.

A sensitive colorimetric high-throughput screening method for lipase synthetic activity assay.

Author information

  • 1College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, People's Republic of China.
  • 2College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, People's Republic of China. Electronic address: hzwangzhao@163.com.

Abstract

A sensitive and practical high-throughput screening method for assaying lipase synthetic activity is described. Lipase-catalyzed transesterification between vinyl acetate and n-butanol in n-hexane was chosen as a model reaction. The released acetaldehyde was determined by the colorimetric method using 3-methyl-2-benzothialinone (MBTH) derivatization. In comparison with other methods, the major advantages of this process include high sensitivity, simple detection, inexpensive reagents, and low requirements for instruments.

Copyright © 2014 Elsevier Inc. All rights reserved.

KEYWORDS:

Derivatization; High-throughput screening; Lipase; MBTH; Synthetic activity

PMID:
24525041
[PubMed - in process]
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