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Springerplus. 2014 Jan 29;3:59. doi: 10.1186/2193-1801-3-59. eCollection 2014.

Effects of cyclic equibiaxial mechanical stretch on α-BK and TRPV4 expression in equine chondrocytes.

Author information

  • 1School of Veterinary Medicine and Science, Faculty of Medicine and Health Sciences, The University of Nottingham, Sutton Bonington Campus, Leicestershire, LE12 5RD UK.
  • 2School of Veterinary Medicine and Science, Faculty of Medicine and Health Sciences, The University of Nottingham, Sutton Bonington Campus, Leicestershire, LE12 5RD UK ; Medical Research Council-Arthritis Research UK Centre for Musculoskeletal Ageing Research, Leicestershire, UK.

Abstract

BACKGROUND:

Chondrocytes are regularly exposed to load-induced stimuli and have the capability to sense and respond to applied mechanical stress. However, the mechanisms involved in chondrocyte mechanotransduction are not clearly understood. The purpose of this study was to explore the effects of cyclic equibiaxial mechanical stretch on the expression of α-BK and TRPV4 channels.

FINDINGS:

Freshly isolated equine articular chondrocytes were subjected to mechanical stress (8% elongation at frequency of 0.5 Hz for 8 h). Western blotting was used to investigate the expression of BKCa and TRPV4 channel proteins. Mechanical stretch increased the expression of BKCa channels by 1.8 fold but TRPV4 expression was not affected.

CONCLUSIONS:

Upregulation of BKCa channel may be the result of direct membrane stretch or elevated intracellular Ca(2+).

KEYWORDS:

BK channel; Chondrocyte; Mechanical stretch; Mechanotransduction; TRPV4

PMID:
24516787
[PubMed]
PMCID:
PMC3913796
Free PMC Article
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