Brilliant Blue G assisted epiretinal membrane surgery

Sci Rep. 2014 Feb 10:4:3956. doi: 10.1038/srep03956.

Abstract

We report intensely staining epiretinal membrane (ERM) with Brilliant Blue G (BBG) under air for two minutes. ERM peeling was performed in 21 cases. After removal of posterior hyaloid, 0.2 mL BBG was first applied on the macula, to stain ERM under air conditions for 2 minutes. Internal limiting membrane (ILM) was intensely stained and peeled in all cases following ERM removal. In 4 cases, the ERM was also observed to be intensely stained with BBG and peeled with an ILM forceps. Postoperatively, the ganglion cell layer thickness was lower in three of the cases, however VA improved in all cases and multifocal electroretinogram revealed no toxicity. Light microscopy of ERM revealed masses of cells whereas; the ILM did not. The increased staining characteristics of ERM and ILM may be resulted from longer contact time of BBG under air pressure.

Publication types

  • Clinical Trial, Phase IV
  • Randomized Controlled Trial

MeSH terms

  • Coloring Agents / therapeutic use
  • Electroretinography
  • Epiretinal Membrane / surgery*
  • Humans
  • Indicators and Reagents / therapeutic use*
  • Macula Lutea / pathology
  • Macula Lutea / surgery
  • Recurrence
  • Retinal Ganglion Cells / drug effects
  • Retinal Ganglion Cells / physiology
  • Rosaniline Dyes / therapeutic use*
  • Staining and Labeling
  • Visual Acuity
  • Vitrectomy

Substances

  • Coloring Agents
  • Indicators and Reagents
  • Rosaniline Dyes
  • coomassie Brilliant Blue