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Biophys J. 2014 Feb 4;106(3):535-47. doi: 10.1016/j.bpj.2013.11.4503.

β1a490-508, a 19-residue peptide from C-terminal tail of Cav1.1 β1a subunit, potentiates voltage-dependent calcium release in adult skeletal muscle fibers.

Author information

  • 1Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland.
  • 2John Curtin School of Medical Research, Australian National University, Canberra, Australia.
  • 3Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland. Electronic address: mschneid@umaryland.edu.

Abstract

The α1 and β1a subunits of the skeletal muscle calcium channel, Cav1.1, as well as the Ca(2+) release channel, ryanodine receptor (RyR1), are essential for excitation-contraction coupling. RyR1 channel activity is modulated by the β1a subunit and this effect can be mimicked by a peptide (β1a490-524) corresponding to the 35-residue C-terminal tail of the β1a subunit. Protein-protein interaction assays confirmed a high-affinity interaction between the C-terminal tail of the β1a and RyR1. Based on previous results using overlapping peptides tested on isolated RyR1, we hypothesized that a 19-amino-acid residue peptide (β1a490-508) is sufficient to reproduce activating effects of β1a490-524. Here we examined the effects of β1a490-508 on Ca(2+) release and Ca(2+) currents in adult skeletal muscle fibers subjected to voltage-clamp and on RyR1 channel activity after incorporating sarcoplasmic reticulum vesicles into lipid bilayers. β1a490-508 (25 nM) increased the peak Ca(2+) release flux by 49% in muscle fibers. Considerably fewer activating effects were observed using 6.25, 100, and 400 nM of β1a490-508 in fibers. β1a490-508 also increased RyR1 channel activity in bilayers and Cav1.1 currents in fibers. A scrambled form of β1a490-508 peptide was used as negative control and produced negligible effects on Ca(2+) release flux and RyR1 activity. Our results show that the β1a490-508 peptide contains molecular components sufficient to modulate excitation-contraction coupling in adult muscle fibers.

Copyright © 2014 Biophysical Society. Published by Elsevier Inc. All rights reserved.

PMID:
24507594
[PubMed - in process]
PMCID:
PMC3944469
[Available on 2015/2/4]
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