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Sex Transm Infect. 2014 Jun;90(4):297-302. doi: 10.1136/sextrans-2013-051229. Epub 2014 Feb 6.

Molecular epidemiology of Neisseria gonorrhoeae isolates from Saskatchewan, Canada: utility of NG-MAST in predicting antimicrobial susceptibility regionally.

Author information

  • 1Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
  • 2Saskatchewan Disease Control Laboratory, Regina, Saskatchewan, Canada.
  • 3Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada Vaccine and Infectious Disease Organization-International Vaccine Centre, University of Saskatchewan, Saskatchewan, Canada.

Abstract

OBJECTIVES:

To investigate the molecular epidemiology of isolates of Neisseria gonorrhoeae from Saskatchewan, Canada, using Neisseria gonorrhoeae multi antigen sequence typing (NG-MAST), and to assess associations between antimicrobial susceptibility (AMS) and specific strain types (STs).

METHODS:

320 consecutive gonococcal isolates, collected between 2003 and 2008, were typed by NG-MAST. STs were grouped if one of their alleles was common and the other differed by ≤1% in DNA sequence. AMS was determined by agar dilution (CLSI) to seven antibiotics.

RESULTS:

N gonorrhoeae isolates were resolved into 82 individual NG-MAST STs and 18 NG-MAST ST groups with groups 25, 3655, 921, 3654, 3657 and 3656 comprising 53.4% (171/320) of the isolates. N gonorrhoeae isolates susceptible to all the tested antimicrobials were significantly (p<0.05) associated with ST 25 (87%). Other significant associations between ST and AMS included: ST 3654 and isolates with minimum inhibitory concentrations of ≥0.03 mg/L to third generation cephalosporins; ST 3711 (100%) and TRNG; and ST/group 3654 (43%) and chromosomal resistance to penicillin and tetracycline. Several NG-MAST STs/groups were significantly associated with isolates with chromosomal resistance to tetracycline. Isolates resistant to ciprofloxacin (n=5) and azithromycin (n=2) appeared as individual STs. Significant associations were observed among individual STs, sex and age of the patient, and regional and temporal distributions.

CONCLUSIONS:

Associations between N gonorrhoeae AMS and NG-MAST STs were identified and may be useful in predicting AMS regionally. Because STs in different countries vary considerably, the use of NG-MAST for the prediction of AMS globally requires further study.

Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

KEYWORDS:

Antibiotic Sensitivity; Molecular Epidemiology; Molecular Typing; Neisseria Gonorrhoea

PMID:
24503900
[PubMed - indexed for MEDLINE]
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