A tuned affinity-based staurosporine probe for in situ profiling of protein kinases

Xiamin Cheng; Lin Li; Mahesh Uttamchandani; Shao Q Yao

doi:10.1039/c4cc00184b

A tuned affinity-based staurosporine probe for in situ profiling of protein kinases

Chem Commun (Camb). 2014 Mar 18;50(22):2851-3. doi: 10.1039/c4cc00184b. Epub 2014 Feb 4.

Authors

Xiamin Cheng¹, Lin Li, Mahesh Uttamchandani, Shao Q Yao

Affiliation

¹ Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore117543. chmyaosq@nus.edu.sg.

PMID: 24496501
DOI: 10.1039/c4cc00184b

Abstract

A clickable and cell-permeable affinity-based probe (AfBP) was designed from staurosporine, by incorporating an electrophilic chloroacetamide warhead to facilitate in situ proteome labeling of a range of potential kinase targets.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acetamides / chemistry
Hep G2 Cells
Humans
Molecular Docking Simulation
Protein Kinase Inhibitors / chemistry*
Protein Kinase Inhibitors / pharmacology
Protein Kinases / chemistry*
Protein Kinases / metabolism
Proteomics
Staurosporine / chemistry*
Staurosporine / pharmacology

Substances

Acetamides
Protein Kinase Inhibitors
chloroacetamide
Protein Kinases
Staurosporine