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PLoS One. 2014 Jan 24;9(1):e84005. doi: 10.1371/journal.pone.0084005. eCollection 2014.

A novel complex A/C/G intergenotypic recombinant of hepatitis B virus isolated in southern China.

Author information

  • 1Department of Biochemistry and Molecular Biology, Guilin Medical University, Guilin, Guangxi, China.
  • 2Viral Hepatitis Research Laboratory, Institute of Infectious Diseases/Liver Failure Medical Center, Beijing 302 Hospital, Beijing, China.
  • 3Division of Hepatology, The Third People's Hospital of Guilin, Guilin, Guangxi, China.
  • 4Guangxi Key Laboratory of Molecular Medicine in Liver Injury and Repair, Guilin Medical University, Guilin, Guangxi, China.

Abstract

Hepatitis B virus (HBV) genotypes and subgenotypes may vary in geographical distribution and virological features. Previous investigations, including ours, showed that HBV genotypes B and C were respectively predominant in South and North China, while genotypes A and D were infrequently detected and genotype G was not found. In this study, a novel A/C/G intergenotype was identified in patients with chronic HBV infection in Guilin, a city in southern China. Initial phylogenetic analysis based on the S gene suggested the HBV recombinant to be genotype G. However, extended genotyping based on the entire HBV genome indicated it to be an A/C/G intergenotype with a closer relation to genotype C. Breakpoint analysis using the SIMPLOT program revealed that the recombinant had a recombination with a arrangement of genotypes A, G, A and C fragments. Compared with the HBV recombinants harboring one or two genotype G fragments found in Asian countries, this Guilin recombinant was highly similar to the Vietnam (98-99%) and Long An recombinants (96-99%), but had a relatively low similarity to the Thailand one (89%). Unlike those with the typical genotype G of HBV, the patients with the Guilin recombinant were seropositive for HBeAg. Moreover, a relatively high HBV DNA viral load (>2 × 10(6) IU/ml) was detected in the patients, and the analysis of viral replication capacity showed that the Guilin recombinant strains had a competent replication capacity similar to genotypes B and C strains. These findings can aid in not only the clarification of the phylogenetic origin of the HBV recombinants with the genotype G fragment found in Asian countries, but also the understanding of the virological properties of these complicated HBV recombinants.

PMID:
24475029
[PubMed - in process]
PMCID:
PMC3901648
Free PMC Article

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