Effect of salts (NaCl and Na2CO3) on callus and suspension culture of Stevia rebaudiana for Steviol glycoside production

Appl Biochem Biotechnol. 2014 Mar;172(6):2894-906. doi: 10.1007/s12010-014-0736-2. Epub 2014 Jan 23.

Abstract

Steviol glycosides are natural non-caloric sweeteners which are extracted from the leaves of Stevia rebaudiana plant. Present study deals the effect of salts (NaCl and Na2CO3) on callus and suspension culture of Stevia plant for steviol glycoside (SGs) production. Yellow-green and compact calli obtained from in vitro raised Stevia leaves sub-cultured on MS medium supplemented with 2.0 mg l(-1) NAA and different concentrations of NaCl (0.05-0.20%) and Na2CO3 (0.0125-0.10%) for 2 weeks, and incubated at 24 ± 1 °C and 22.4 μmol m(-2) s(-1) light intensity provided by white fluorescent tubes for 16 h. Callus and suspension biomass cultured on salts showed less growth as well as browning of medium when compared with control. Quantification of SGs content in callus culture (collected on 15th day) and suspension cultures (collected at 10th and 15th days) treated with and without salts were analyzed by HPLC. It was found that abiotic stress induced by the salts increased the concentration of SGs significantly. In callus, the quantity of SGs got increased from 0.27 (control) to 1.43 and 1.57% with 0.10% NaCl, and 0.025% Na2CO3, respectively. However, in case of suspension culture, the same concentrations of NaCl and Na2CO3 enhanced the SGs content from 1.36 (control) to 2.61 and 5.14%, respectively, on the 10th day.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomass
  • Carbonates / pharmacology*
  • Chromatography, High Pressure Liquid
  • Diterpenes, Kaurane / biosynthesis*
  • Diterpenes, Kaurane / isolation & purification
  • Light
  • Plant Leaves / cytology
  • Plant Leaves / drug effects*
  • Plant Leaves / metabolism
  • Plant Somatic Embryogenesis Techniques
  • Sodium Chloride / pharmacology*
  • Stevia / cytology
  • Stevia / drug effects*
  • Stevia / metabolism
  • Sweetening Agents
  • Temperature

Substances

  • Carbonates
  • Diterpenes, Kaurane
  • Sweetening Agents
  • Sodium Chloride
  • sodium carbonate
  • steviol