Format

Send to:

Choose Destination
See comment in PubMed Commons below
Genet Mol Res. 2014 Jan 10;13(1):117-26. doi: 10.4238/2014.January.10.2.

Molecular cloning, expression, and regulation of the ovalbumin gene in pigeon oviduct epithelial cells.

Author information

  • 1College of Animal Sciences and Technology, Nanjing Agriculture University, Nanjing, Jiangsu, China.
  • 2Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, China.
  • 3PingYang XingLiang Pigeon Farming Co. Ltd., Wenzhou, Zhejiang, China.
  • 4College of Animal Sciences and Technology, Nanjing Agriculture University, Nanjing, Jiangsu, China yanps@njau.edu.cn.

Abstract

The full-length pigeon ovalbumin (OVA) gene cDNA was cloned and sequenced by reverse transcription-polymerase chain reaction (RT-PCR) and rapid-amplification of cDNA ends. A 386-amino acid protein was predicted for the obtained sequence, which had 67% identity with the chicken protein. Similar to chicken OVA, the pigeon OVA gene is a non-inhibitory serine protease inhibitor. Quantitative PCR analysis revealed that pigeon OVA mRNA was highly expressed in the oviduct, and trace amounts were detected in other tissues. During the reproductive cycle, pigeon oviduct OVA mRNA expression reached its peak during the egg-laying stage, decreased with brooding, and then increased again during the squab-feeding period. Moreover, the relative OVA expression level in pigeon oviduct epithelial cells could be upregulated by a constant concentration of steroid hormones.

PMID:
24446294
[PubMed - indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Fundacao de Pesquisas Cientificas de Ribeirao Preto
    Loading ...
    Write to the Help Desk