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Dent Mater. 2014 Mar;30(3):249-62. doi: 10.1016/j.dental.2013.11.014. Epub 2014 Jan 17.

Remineralization potential of fully demineralized dentin infiltrated with silica and hydroxyapatite nanoparticles.

Author information

  • 1School of Clinical Dentistry, University of Sheffield, Sheffield, UK. Electronic address: alexander.besinis@plymouth.ac.uk.
  • 2School of Clinical Dentistry, University of Sheffield, Sheffield, UK.

Abstract

OBJECTIVE:

This study investigates the potential of a novel guided tissue regeneration strategy, using fully demineralized dentin infiltrated with silica and hydroxyapatite (HA) nanoparticles (NPs), to remineralize dentin collagen that is completely devoid of native hydroxyapatite.

METHODS:

Dentin blocks were fully demineralized with 4N formic acid and subsequently infiltrated with silica and HA NPs. The remineralizing potential of infiltrated dentin was assessed following a twelve week exposure to an artificial saliva solution by means of TEM, EDS and micro-CT. Measurements were taken at baseline and repeated at regular intervals for the duration of the study to quantify the P and Ca levels, the mineral volume percentage and mineral separation of the infiltrated dentin specimens compared to sound dentin and non-infiltrated controls.

RESULTS:

Infiltration of demineralized dentin with nano-HA restored up to 55% of the P and Ca levels at baseline. A local increase in the concentration of calcium phosphate compounds over a period of twelve weeks resulted in a higher concentration in P and Ca levels within the infiltrated specimens when compared to the non-infiltrated controls. Remineralization of demineralized dentin with silica NPs by immersion in artificial saliva was the most effective strategy, restoring 20% of the P levels of sound dentin. Micro-CT data showed a 16% recovery of the mineral volume in dentin infiltrated with silica NPs and a significant decrease in the mineral separation to levels comparable to sound dentin.

SIGNIFICANCE:

Demineralized dentin infiltrated with silica NPs appears to encourage heterogeneous mineralization of the dentin collagen matrix following exposure to an artificial saliva solution.

Copyright © 2013 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

KEYWORDS:

Artificial saliva; Calcium phosphates; Collagen; Colloidal silica; Demineralization; Dentin infiltration; Hydroxyapatite; Nano-HA; Nanoparticles; Remineralization

PMID:
24444789
[PubMed - in process]
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