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Int J Nanomedicine. 2014;9:379-88. doi: 10.2147/IJN.S53546. Epub 2014 Jan 7.

Rapid green synthesis of silver nanoparticles from Chrysanthemum indicum L and its antibacterial and cytotoxic effects: an in vitro study.

Author information

  • 1Department of Animal Nutrition and Physiology, National Institute of Animal Science, Rural Development Administration, Suwon, Republic of Korea.
  • 2Grassland and Forage Division, National Institute of Animal Science, Rural Development Administration, Seonghwan-Eup, Cheonan-Si, Chungnam, Republic of Korea.
  • 3Center for Environmental Research and Development, Loyola College, Chennai, India.
  • 4Research and Development, Vel Tech Dr RR and Dr SR Technical University, Chennai, India.
  • 5Department of Animal Science, Chungbuk National University, Chungbuk, Republic of Korea.
  • 6Department of Animal Nutrition and Physiology, National Institute of Animal Science, Rural Development Administration, Suwon, Republic of Korea ; Department of Animal Science, Seoul National University, Pyeongchang, Republic of Korea.

Abstract

The present work reports a simple, cost-effective, and ecofriendly method for the synthesis of silver nanoparticles (AgNPs) using Chrysanthemum indicum and its antibacterial and cytotoxic effects. The formation of AgNPs was confirmed by color change, and it was further characterized by ultraviolet-visible spectroscopy (435 nm). The phytochemical screening of C. indicum revealed the presence of flavonoids, terpenoids, and glycosides, suggesting that these compounds act as reducing and stabilizing agents. The crystalline nature of the synthesized particles was confirmed by X-ray diffraction, as they exhibited face-centered cubic symmetry. The size and morphology of the particles were characterized by transmission electron microscopy, which showed spherical shapes and sizes that ranged between 37.71-71.99 nm. Energy-dispersive X-ray spectroscopy documented the presence of silver. The antimicrobial effect of the synthesized AgNPs revealed a significant effect against the bacteria Klebsiella pneumonia, Escherichia coli, and Pseudomonas aeruginosa. Additionally, cytotoxic assays showed no toxicity of AgNPs toward 3T3 mouse embryo fibroblast cells (25 μg/mL); hence, these particles were safe to use.

KEYWORDS:

Chrysanthemum indicum; antibacterial activity; cytotoxic; green synthesis; silver nanoparticle

PMID:
24426782
[PubMed - indexed for MEDLINE]
PMCID:
PMC3890422
Free PMC Article
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