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Cell Immunol. 2014 Feb;287(2):74-7. doi: 10.1016/j.cellimm.2013.12.007. Epub 2013 Dec 21.

Interleukin-17F attenuates H2O2-induced cell cycle arrest.

Author information

  • 1School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China. Electronic address: ztong@whu.edu.cn.
  • 2Zhongshan Hospital of Hubei Province, Wuhan 430033, China.
  • 3School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China.

Abstract

Interleukin IL-17F was expressed in colon epithelial cells and showed multiple functions in colon tumorigenesis. However, the role of IL-17F in colon cancer cell cycle progression remains unclear. In this study, we analyzed the effects of IL-17F on oxidant-induced cell cycle shift in human colon cancer cells. IL-17F overexpressing and wildtype HCT116 cells were challenged with H(2)O(2). Cell cycle distribution analysis showed IL-17F attenuated H(2)O(2)-induced G2/M phase arrest by inhibiting S to G2/M transition. We further checked expression levels of two critical cell cycle regulators p21 and p27. The results showed that IL-17F could inhibit H(2)O(2) induced p27 up-regulation. Meanwhile, IL-17F could increase the phosphorylation of p38 after H(2)O(2) treatment. The regulations of p27 level and p38 activity may contribute to the impaired G2/M phase arrest by IL-17F. Taken together, our findings extend IL-17F as an important factor in colon cancer development and provide new insight into the signaling pathway.

Copyright © 2013 Elsevier Inc. All rights reserved.

KEYWORDS:

Cell cycle; H(2)O(2); Interleukin-17F; p27; p38

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