Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Biomaterials. 2014 Mar;35(9):2664-79. doi: 10.1016/j.biomaterials.2013.11.078. Epub 2014 Jan 8.

Three-dimensional scaffolds of acellular human and porcine lungs for high throughput studies of lung disease and regeneration.

Author information

  • 1University of Vermont, Department of Medicine, 226 Health Sciences, Research Facility Center, Burlington, VT 05405, USA. Electronic address: Darcy.wagner@med.uvm.edu.
  • 2University of Vermont, Department of Medicine, 226 Health Sciences, Research Facility Center, Burlington, VT 05405, USA. Electronic address: Nicholas.Bonenfant@med.uvm.edu.
  • 3University of Vermont, Department of Medicine, 226 Health Sciences, Research Facility Center, Burlington, VT 05405, USA. Electronic address: Dino.Sokocevic@med.uvm.edu.
  • 4University of Vermont, Department of Medical Biostatistics, 27 Hills Building, Burlington, VT 05405, USA. Electronic address: mdesarno@uvm.edu.
  • 5University of Vermont, Department of Medicine, 226 Health Sciences, Research Facility Center, Burlington, VT 05405, USA. Electronic address: zachary.borg@med.uvm.edu.
  • 6University of Vermont, Department of Surgery, Fletcher House 301, Burlington, VT 05405, USA. Electronic address: charles.parsons@vtmednet.org.
  • 7University of Vermont, Department of Medicine, 226 Health Sciences, Research Facility Center, Burlington, VT 05405, USA. Electronic address: elice.brooks@med.uvm.edu.
  • 8University of Vermont, Department of Surgery, Fletcher House 301, Burlington, VT 05405, USA. Electronic address: Joseph.Platz@med.uvm.edu.
  • 9University of Arizona, Department of Surgery, 1501 North Campbell Avenue, Tucson, AZ 85724, USA. Electronic address: zkhalpey@surgery.arizona.edu.
  • 10Washington University in St. Louis, Department of Surgery, 1 Barnes Jewish Plaza, 3108 Queeny Tower, St. Louis, MO 63110, USA. Electronic address: hogansondm@wudosis.wustl.edu.
  • 11University of Vermont, Department of Biology, 311 Marsh Life Sciences, Burlington, VT 05405, USA. Electronic address: bdeng@uvm.edu.
  • 12University of Vermont, Department of Biology, 311 Marsh Life Sciences, Burlington, VT 05405, USA. Electronic address: Ying-Wai.Lam@uvm.edu.
  • 13University of Vermont, College of Engineering and Mathematics, 301 Votey Hall, 33 Colchester Ave, Burlington, VT 05405, USA. Electronic address: oldinski@uvm.edu.
  • 14University of Vermont, Department of Medical Biostatistics, 27 Hills Building, Burlington, VT 05405, USA. Electronic address: Takamaru.Ashikaga@uvm.edu.
  • 15University of Vermont, Department of Medicine, 226 Health Sciences, Research Facility Center, Burlington, VT 05405, USA. Electronic address: dweiss@uvm.edu.

Abstract

Acellular scaffolds from complex whole organs such as lung are being increasingly studied for ex vivo organ generation and for in vitro studies of cell-extracellular matrix interactions. We have established effective methods for efficient de and recellularization of large animal and human lungs including techniques which allow multiple small segments (∼ 1-3 cm(3)) to be excised that retain 3-dimensional lung structure. Coupled with the use of a synthetic pleural coating, cells can be selectively physiologically inoculated via preserved vascular and airway conduits. Inoculated segments can be further sliced for high throughput studies. Further, we demonstrate thermography as a powerful noninvasive technique for monitoring perfusion decellularization and for evaluating preservation of vascular and airway networks following human and porcine lung decellularization. Collectively, these techniques are a significant step forward as they allow high throughput in vitro studies from a single lung or lobe in a more biologically relevant, three-dimensional acellular scaffold.

Copyright © 2013 Elsevier Ltd. All rights reserved.

KEYWORDS:

Acellular matrix; Endothelial cell; Epithelial cell; Extracellular matrix (ECM); Human lung fibroblast; Mesenchymal stem cell

PMID:
24411675
[PubMed - indexed for MEDLINE]
PMCID:
PMC4215726
[Available on 2015/3/1]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk