Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Anal Biochem. 2014 Apr 1;450:46-8. doi: 10.1016/j.ab.2013.12.035. Epub 2014 Jan 7.

Quantification analysis of yeast-displayed lipase.

Author information

  • 1Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China.
  • 2Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China. Electronic address: feylin@scut.edu.cn.

Abstract

We describe a method for quantification of displayed lipase on yeast cell surface. The strategy uses an organophosphonate ester to irreversibly inhibit the active lipase and release a detectable fluorescent group. The amount of displayed lipase can be represented as "g/g cell" or "molecules/cell". The results obtained correlated well with those obtained by existing methods. Therefore, this method is credible and will provide a powerful tool to promote research of lipase yeast surface display.

Copyright © 2014 Elsevier Inc. All rights reserved.

KEYWORDS:

Active-site titration; Lipase; Quantification; Yeast surface display

PMID:
24406905
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk