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    Anal Biochem. 1987 Apr;162(1):156-9.

    Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.

    Chomczynski P, Sacchi N.

    Abstract

    A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is particularly useful for processing large numbers of samples and for isolation of RNA from minute quantities of cells or tissue samples.

    PMID:
    2440339
    [PubMed - indexed for MEDLINE]

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