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Diagn Microbiol Infect Dis. 2014 Mar;78(3):249-54. doi: 10.1016/j.diagmicrobio.2013.11.019. Epub 2013 Nov 27.

Evaluation of Brugia malayi sheath protein (Shp-1) as a diagnostic antigen for human lymphatic filariasis.

Author information

  • 1Centre for Biotechnology, Anna University, Chennai, Tamil Nadu, India. Electronic address: jai.auc@gmail.com.
  • 2Centre for Biotechnology, Anna University, Chennai, Tamil Nadu, India. Electronic address: jairavibt@gmail.com.
  • 3Department of Biochemistry, Indian Institute of Science, Bangalore, Karnataka, India. Electronic address: radhikans@gmail.com.
  • 4Centre for Biotechnology, Anna University, Chennai, Tamil Nadu, India. Electronic address: vicchuans@gmail.com.
  • 5Department of Biochemistry, Indian Institute of Science, Bangalore, Karnataka, India. Electronic address: anjali@biochem.iisc.ernet.in.
  • 6Centre for Biotechnology, Anna University, Chennai, Tamil Nadu, India. Electronic address: pkaliraj@annauniv.edu.

Abstract

Lymphatic filariasis is the second leading cause of permanent long-term disability globally and control of this disease needs efficient diagnostic methods. In this study, abundantly expressing microfilarial sheath protein (Shp-1) from Brugia malayi was characterized as a filarial diagnostic candidate using samples from different clinical population. Monoclonal antibodies were developed against E. coli expressed recombinant Shp-1 in order to assess its efficiency in filarial antigen detection assay system. Endemic Normal (EN, n = 170), asymptomatic microfilaeremics (MF, n = 65), symptomatic chronic pathology (CP, n = 45) and non endemic normal (NEN, n = 10) sera were analyzed by antigen capture enzyme-linked immunosorbent assay. Of the 290 individuals, all MF individuals (both brugian and bancroftian) were positive in this assay followed by CP and EN. When compared with SXP-1 and Og4C3 antigen assays, all assays detected Wb MF correctly, Bm MF was detected by Shp-1 and SXP-1 assays, and only Shp-1 was able to detect EN (12%) and CP (29%). Results showed that this assay may be useful for monitoring prior to mass drug administration.

Copyright © 2014 Elsevier Inc. All rights reserved.

KEYWORDS:

Brugia malayi; ELISA; Lymphatic filariasis; Monoclonal antibodies; Sheath protein; Shp-1; Wuchereria bancrofti

[PubMed - indexed for MEDLINE]
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