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J Dermatol Sci. 2014 Apr;74(1):88-92. doi: 10.1016/j.jdermsci.2013.11.013. Epub 2013 Dec 16.

Simple and rapid detection of HLA-A*31:01 for prediction of carbamazepine-induced hypersensitivity using loop-mediated isothermal amplification method.

Author information

  • 1Department of Dermatology, Shimane University Hospital, Izumo, Japan. Electronic address: ofcourse@med.shimane-u.ac.jp.
  • 2Center for Community-Based Health Research and Education (COHRE), Organization for the Promotion of Project Research, Shimane University, Izumo, Japan.
  • 3Department of Pediatrics, Shimane University Hospital, Izumo, Japan.
  • 4Department of Dermatology, Shimane University Hospital, Izumo, Japan.
  • 5Section of Nephrology, Department of Internal Medicine, Shimane University Hospital, Izumo, Japan.
  • 6Section of Cardiovascular Internal Medicine, Department of Internal Medicine, Shimane University Hospital, Izumo, Japan.
  • 7Department of Psychiatry, Shimane University Hospital, Izumo, Japan.



Carbamazepine (CBZ), which is widely used in management of epilepsy or neuropathic pain, causes fatal severe cutaneous adverse reactions (SCARs). CBZ-induced SCARs are known to occur in strong association with human leukocyte antigen (HLA)-A*31:01 in Japanese and European populations. HLA genotyping is currently used to detect human HLA-A*31:01.


To establish a simple and rapid screening assay specific for HLA-A*31:01, the loop-mediated isothermal amplification (LAMP) method was employed on a sample Japanese population.


A set of LAMP primers targeting exon 2 of HLA-A*31:01 were designed. Thirty-two clinical samples including the representative HLA-A allele in Japan were used to assess the specificity of LAMP primers in the detection of HLA-A*31:01.


The HLA-A*31:01-specific LAMP assay showed consistency with polymerase chain reaction reverse sequence-specific oligonucleotide probe (PCR-rSSO) and polymerase chain reaction-sequence based typing (PCR-SBT) results.


High sensitivity and specificity of the HLA-A*31:01 LAMP assay was confirmed. Considering its convenience, the assay can be widely used to screen patients at high genetic risk of CBZ-induced SCARs.

Copyright © 2014 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.


Carbamazepine; Fatal severe cutaneous adverse reactions; Human leukocyte antigen-A*31:01; Loop-mediated isothermal amplification

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