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Anal Biochem. 2014 Mar 15;449:129-31. doi: 10.1016/j.ab.2013.12.030. Epub 2013 Dec 28.

Minimizing artifactual elevation of lipid peroxidation products (F2-isoprostanes) in plasma during collection and storage.

Author information

  • 1School of Medicine & Pharmacology, Royal Perth Hospital, University of Western Australia, Perth, WA 6000, Australia. Electronic address: anne.barden@uwa.edu.au.
  • 2School of Medicine & Pharmacology, Royal Perth Hospital, University of Western Australia, Perth, WA 6000, Australia.
  • 3West Australian Institute for Medical Research, University of Western Australia, Perth, WA 6000, Australia.

Abstract

F2-isoprostanes (F2-IsoP's) are reliable measures of in vivo lipid oxidation, but care is required to prevent artifactual elevation. We examined the effects of blood collection and storage on plasma F2-IsoP's. Blood was collected into EDTA/butylated hydroxytoluene/reduced glutathione (EDTA/BHT/GSH) or EDTA, at 4 °C or room temperature. Plasma was stored at -20 or -80 °C for 1 or 6 months before F2-IsoP's were assayed by GC-MS. The temperature of blood collection did not affect F2-IsoP's. However, storage at -20 °C or collection into EDTA resulted in significant increases in F2-IsoP's. Blood collection into EDTA/BHT/GSH and storage at -80 °C minimizes artifactual elevation of plasma F2-IsoP's.

Copyright © 2014 Elsevier Inc. All rights reserved.

KEYWORDS:

Gas chromatography–mass spectrometry; Isoprostanes; Lipid peroxidation

PMID:
24380789
[PubMed - in process]
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