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FEBS Lett. 2014 Jan 31;588(3):466-71. doi: 10.1016/j.febslet.2013.12.008. Epub 2013 Dec 24.

Rre37 stimulates accumulation of 2-oxoglutarate and glycogen under nitrogen starvation in Synechocystis sp. PCC 6803.

Author information

  • 1Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 657-8501, Japan.
  • 2Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 657-8501, Japan.
  • 3Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 657-8501, Japan; Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency, 3-5 Sanbancho, Chiyoda-ku, Tokyo 102-0075, Japan.
  • 4RIKEN Biomass Engineering Program, 1-7-22, Suehirocho, Tsurumi, Yokohama 230-0045, Japan; Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University,1-5 Yamadaoka, Suita, Osaka 565-0871, Japan.
  • 5PRESTO, Japan Science and Technology Agency (JST), 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan.
  • 6RIKEN Center for Sustainable Resource Science, 1-7-22, Suehirocho, Tsurumi, Yokohama 230-0045, Japan.
  • 7Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 657-8501, Japan; RIKEN Biomass Engineering Program, 1-7-22, Suehirocho, Tsurumi, Yokohama 230-0045, Japan. Electronic address: akondo@kobe-u.ac.jp.

Abstract

Rre37 (sll1330) in a cyanobacterium Synechocystis sp. PCC 6803 acts as a regulatory protein for sugar catabolic genes during nitrogen starvation. Low glycogen accumulation in Δrre37 was due to low expression of glycogen anabolic genes. In addition to low 2-oxoglutarate accumulation, normal upregulated expression of genes encoding glutamate synthases (gltD and gltB) as well as accumulation of metabolites in glycolysis (fructose-6-phosphate, fructose-1,6-bisphosphate, and glyceraldehyde-3-phosphate) and tricarboxylic acid (TCA) cycle (oxaloacetate, fumarate, succinate, and aconitate) were abolished by rre37 knockout. Rre37 regulates 2-oxoglutarate accumulation, glycogen accumulation through expression of glycogen anabolic genes, and TCA cycle metabolites accumulation.

Copyright © 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

KEYWORDS:

2-OG; 2-Oxoglutarate; 2-oxoglutarate; Cyanobacteria; GOGAT; GS; Glycogen; HPLC; LC/QqQ-MS; Nitrogen starvation; OD; Rre37; TCA; glutamate synthase; glutamine synthetase; high performance liquid chromatography; liquid chromatography/triple quadrupole mass spectrometry; optical density; tricarboxylic acid

PMID:
24374346
[PubMed - indexed for MEDLINE]
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