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Biosens Bioelectron. 2014 May 15;55:141-8. doi: 10.1016/j.bios.2013.11.075. Epub 2013 Dec 10.

Effects of nanoparticle size and cell type on high sensitivity cell detection using a localized surface plasmon resonance biosensor.

Author information

  • 1Department of Electronic Engineering, City University of Hong Kong, Kowloon, Hong Kong; Center for Biosystems, Neuroscience, and Nanotechnology, City University of Hong Kong, Kowloon, Hong Kong; Department of Electronic Information Engineering, Tianjin University, Tianjin 300072, China.
  • 2Department of Biology and Chemistry, City University of Hong Kong, Kowloon, Hong Kong; Center for Biosystems, Neuroscience, and Nanotechnology, City University of Hong Kong, Kowloon, Hong Kong.
  • 3Department of Physics and Materials Science, City University of Hong Kong, Kowloon, Hong Kong; Center for Biosystems, Neuroscience, and Nanotechnology, City University of Hong Kong, Kowloon, Hong Kong.
  • 4Department of Electronic Engineering, City University of Hong Kong, Kowloon, Hong Kong; Center for Biosystems, Neuroscience, and Nanotechnology, City University of Hong Kong, Kowloon, Hong Kong. Electronic address: pang@cityu.edu.hk.

Abstract

A localized surface plasmon resonance (LSPR) effect was used to distinguish cell concentration on ordered arrays of Au nanoparticles (NPs) on glass substrates. Human-derived retinal pigment epithelial RPE-1 cells with flatter bodies and higher confluency were compared with breast cancer MCF-7 cells. Nanosphere lithography was used to form Au NPs with average diameters of 500 and 60 nm in order to compare cell detection range, resonance peak shift, and cell concentration sensitivity. A larger cell concentration range was detected on the larger 500 nm Au NPs compared to 60 nm Au NPs (8.56 × 10(3)-1.09 × 10(6) vs. 3.43 × 10(4)-2.73 × 10(5)cells/ml). Resonance peak shift could distinguish RPE-1 from MCF-7 cells on both Au NPs. RPE-1 cells consistently displayed larger resonance peak shifts compared to MCF-7 cells until the detection became saturated at higher concentration. For both types of cells, higher concentration sensitivity in the range of ~10(4)-10(6)cells/ml was observed on 500 nm compared to 60 nm Au NPs. Our results show that cells on Au NPs can be detected in a large range and at low concentration. Optimal cell sensing can be achieved by altering the dimensions of Au NPs according to different cell characteristics and concentrations.

© 2013 Published by Elsevier B.V.

KEYWORDS:

Au nanoparticles; Breast cancer MCF-7 cell; Cell concentration detection; Human-derived retinal pigment epithelial RPE-1 cell; Localized surface plasmon resonance (LSPR); Resonance peak shift

PMID:
24373953
[PubMed - in process]
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