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Biomed Pharmacother. 2014 Apr;68(3):377-84. doi: 10.1016/j.biopha.2013.10.011. Epub 2013 Dec 2.

Annexin A7 and its binding protein galectin-3 influence mouse hepatocellular carcinoma cell line in vitro.

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  • 1The Key laboratory of tumor metastasis in Liaoning Province, Dalian Medical University, 9 West, Lvshun Southern Road, Dalian 116044, China.
  • 2The Key laboratory of tumor metastasis in Liaoning Province, Dalian Medical University, 9 West, Lvshun Southern Road, Dalian 116044, China. Electronic address: lilianhong@dlmedu.edu.cn.
  • 3The Key laboratory of tumor metastasis in Liaoning Province, Dalian Medical University, 9 West, Lvshun Southern Road, Dalian 116044, China. Electronic address: jwtang_53@sina.cn.

Abstract

Lymph node metastasis is recognized as an important mode of liver cancer metastasis. Our previous study has built two hepatocarcinoma cell lines, Hca-F with high (75%) and Hca-P with low (25%) incidences of lymph node metastasis, and has indicated that annexin A7 is an important factor in the lymphatic metastasis of tumors. There is evidence that galectin-3 is the binding protein of annexin A7 and works in protein complexes. Our current study shows that both annexin A7 and galectin-3 express higher in Hca-F than Hca-P. Annexin A7 was successfully down-regulated in Hca-P by RNA interference, and this resulted in concomitant reduction of galactin 3 expression in annexin A7 down regulated compared to the control and N-control cells. Using CCK-8 assay, the expression level of annexin A7 and galectin-3 were found to have correlation with the proliferation ability; Transwell assay showed annexin A7 and galectin-3 are involved in cell migration and invasion regulation in mouse hepatocellular carcinoma cell lines, immunofluorescence assay indicate annexin A7 and galectin-3 were co-located annexin A7 and galectin-3 played roles in DNA damage and cell proliferation cycle checkpoint arrest pathway. Those phenomena indicated that annexin A7 influences lymphatic metastasis of tumors by interacting with galectin-3 through the regulation of tumor cell proliferation, attachment, migration and invasion.

Copyright © 2013 Elsevier Masson SAS. All rights reserved.

KEYWORDS:

Annexin A7; Binding protein; Galectin-3; Hepatocarcinoma; Lymphatic metastasis

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