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Curr Microbiol. 2014 May;68(5):587-93. doi: 10.1007/s00284-013-0513-4. Epub 2013 Dec 27.

Combination of culture-dependent and -independent methods reveals diverse acyl homoserine lactone-producers from rhizosphere of wetland plants.

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  • 1Department of Environmental Science, College of Environmental and Resource Sciences, Zhejiang University, Room B470, 688 Yu Hang Tang Road, Hangzhou, 310058, China.


The culture-dependent method and a degenerate primer-based culture-independent method were combined in an effort to identify N-acyl homoserine lactone (AHL) producers in rhizosphere of wetland plants, Salix babylonica (willow) and Phragmites australis (reed). Overall, eight potential AHL-producing genera were found, which were Aeromonas, Pseudomonas, Polymorphum, Agrobacterium, Rhizobium, Sinorhizobium, Ensifer, and Pectobacterium. Thin layer chromatograph assay revealed various AHL profiles from cultivable AHL-producers. The degenerate primer pair RAHL352F and RAHL461R was found to cover AHL synthetase genes from families Rhizobiaceae and Rhodobacteraceae. Little overlap was found in taxa of potential AHL-producers obtained by the two methods, indicating that they were well complement to each other. This is the first survey for AHL-producers that employed combined culture-dependent and -independent methods.

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