Myelin basic protein (MBP) is a major protein component of myelin sheath. Primarily because of its ability to induce experimental allergic encephalomyelitis (EAE) in animals, this protein has been considered to play an important role in the pathogenesis of multiple sclerosis (MS), which is one of the most common demyelinating diseases. Its precise measurement in the cerebrospinal fluid (CSF) has been uncommonly difficult, mainly due to immunoheterogeneity of MBP or MBP-like material. More recently, highly sensitive radioimmunoassay techniques utilizing well-characterized antisera have been developed that facilitated its use in the management of MS. The clinical course of MS is highly variable, and the disease is characterized by periods of remission and relapses. Many studies have demonstrated the release of MBP during relapses and elevated levels of MBP in the CSF can be detected if lumbar puncture is performed within 7 days of the onset of neurologic symptoms suggestive of MS. However, the presence of MBP is not an absolute indicator of MS, as elevated MBP levels are also frequently observed in other demyelinating diseases.