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Mutat Res Genet Toxicol Environ Mutagen. 2014 Jan 1;759:28-36. doi: 10.1016/j.mrgentox.2013.07.018. Epub 2013 Dec 17.

Mutagenic effects of carbon-ion irradiation on dry Arabidopsis thaliana seeds.

Author information

  • 1Department of Biophysics, Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Road, Lanzhou 730000, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
  • 2Department of Biophysics, Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Road, Lanzhou 730000, PR China.
  • 3Department of Biophysics, Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Road, Lanzhou 730000, PR China; School of Pharmacy, Lanzhou University, 199 West Donggang Road, Lanzhou 730020, PR China.
  • 4Institute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou 310029, PR China.
  • 5Department of Biophysics, Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Road, Lanzhou 730000, PR China. Electronic address: libinzhou@impcas.ac.cn.

Abstract

To investigate the mutagenic effects of carbon ions on Arabidopsis thaliana (ecotype Columbia) and to isolate useful genes in plant development, dry seeds were exposed to 43MeV/u carbon ions at doses of 0, 100, 200, 300, 400, 500 and 600Gy. The survival rate, primary root length, and hypocotyl length of M1 plants were analyzed, and 200Gy was selected as the dose for the large-scale experiment. A total of 1363 lines of plants from 28,062 M2 populations displayed alterations in the leaf, stem, flower, or life cycle, with abnormal leaves and a premature life cycle as the main phenotypic variations. The mutated gene loci of five stable and inheritable mutations were roughly mapped on chromosomes. Novel mutants were obtained, although some of the mutants were similar to mutants induced by ethylmethane sulfonate (EMS) according to previous studies. This study provides a large body of specific information describing A. thaliana mutation phenotypes that were induced by carbon-ion irradiation. These results suggest that carbon-ion beams are as useful and effective as other mutagens for mutant breeding in plants, and that they will allow mutant breeding that is more diversified.

Copyright © 2013 Elsevier B.V. All rights reserved.

KEYWORDS:

Arabidopsis thaliana; Carbon ion beams; Irradiation; Mutation breeding

PMID:
24361377
[PubMed - indexed for MEDLINE]
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