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Chin J Nat Med. 2013 Sep;11(5):572-6. doi: 10.1016/S1875-5364(13)60103-X.

Analysis of isorhamnetin-3-O-neohesperidoside in rat plasma by liquid chromatography/electrospray ionization tandem mass spectrometry and its application to pharmacokinetic studies.

Author information

  • 1Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.
  • 2Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China.
  • 3Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China. Electronic address: snow8185@yahoo.com.cn.
  • 4State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China. Electronic address: boyangyu59@163.com.

Abstract

AIM:

To establish an LC-MS/MS method for determination of isorhamnetin-3-O-neohesperidoside and investigate its application on pharmacokinetic study in rats.

METHODS:

Eight rats were given 5 mg·kg(-1) isorhamnetin-3-O-neohesperidoside after intravenous administration. A highly sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for the determination of isorhamnetin-3-O-neohesperidosidein rat plasma using rutin as internal standard. The analytes and rutin (internal standard) were extracted with methanol followed by a rapid isocratic elution with 10 mmol·L(-1) ammonium acetate buffer/methanol (20 : 80, V/V) on a C18 column (150 mm × 2.1 mm, I.D., 5 μm) and subsequent analysis by mass spectrometry in the multi-eaction-monitoring mode.

RESULTS:

The assays were linear over the concentration range of 0.01-10 μg·mL(-1) for isorhamnetin-3-O-neohesperidosidein rat plasma. The lower limit of quantifications for isorhamnetin-3-O-neohesperidoside was 0.01 μg·mL(-1).

CONCLUSION:

The validated method is successfully applied to determine the plasma concentrations of isorhamnetin-3-O-neohesperidosidein in rats.

Copyright © 2013 China Pharmaceutical University. Published by Elsevier B.V. All rights reserved.

KEYWORDS:

Electrospray ionization; Isorhamnetin-3-O-neohesperidoside; LC-MS/MS; Pharmacokinetics; Rat plasma

PMID:
24359786
[PubMed - indexed for MEDLINE]
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