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[Comparison of ELISA and pharmacia UNICAP detection reagent for house dust mite specific IgG4].

[Article in Chinese]

Author information

  • 1Jiangsu Huaguan Biotechnology Joint Stock Company Ltd, Taizhou, 225300, China.
  • 2Hubei Extny Exit Inspection and Quarantine Bureau.
  • 3Department of Allergy, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology.
  • 4Department of Otorhinolaryngology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology.

Abstract

OBJECTIVE:

To compare the ELISA and Pharmica unicap reagents of Dermatophagoides pteronyssinus specific IgG4 in clinic.

METHOD:

The stability of ELISA reagent was studied first with parameters of intra and inter assay variations (%CV). Calibration curve of the two reagents was compared. Sixty-five serum samples before or after standardized specific immunotherapy were examined with the two methods, correlation coefficient was calculated.

RESULT:

The stability of ELISA reagent was good with low intra and inter assay variations (3.48% and 5.78%). Calibration curve of the two reagents was similar. The correlation coefficient was 0.93 between the two test methods.

CONCLUSION:

The results of ELISA and Pharmica unicap reagents of Dermatophagoides pteronyssinus specific IgG4 were highly consistent. The ELISA reagents could be applied in clinic.

PMID:
24358790
[PubMed - indexed for MEDLINE]
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