-
Cloning and expression of cDNA encoding human basic fibroblast growth factor.
A cDNA encoding human basic fibroblast growth factor (bFGF) was isolated from a human foreskin fibroblast cDNA library. The cDNA, 4 kilobases in size, had a coding sequence, 5' and 3' untranslated regions, and a poly(A) chain. Isolation of additional cDNA clones that had a short 3' untranslated region suggested the presence of multiple mRNA forms. By Northern blot analysis, at least five bFGF mRNA species were detected in cultured fibroblast cells. Transfection of the cDNA to COS cells resulted in the detection of mitogenic activity in the culture medium of the transfected cells, suggesting that a part of the synthesized protein might be secreted from cells despite its unusual short signal sequence.
PMID: 2435575 [PubMed - indexed for MEDLINE]
-
Cited by 16 PubMed Central articles
-
Mapping protein-protein interactions by affinity-directed mass spectrometry.
Zhao Y, Muir TW, Kent SB, Tischer E, Scardina JM, Chait BT.
Proc Natl Acad Sci U S A. 1996 Apr 30; 93(9):4020-4.
[Proc Natl Acad Sci U S A. 1996]
-
Molecular cloning of a novel cytokine cDNA encoding the ninth member of the fibroblast growth factor family, which has a unique secretion property.
Miyamoto M, Naruo K, Seko C, Matsumoto S, Kondo T, Kurokawa T.
Mol Cell Biol. 1993 Jul; 13(7):4251-9.
[Mol Cell Biol. 1993]
-
Regulation of bFGF gene expression and subcellular distribution of bFGF protein in adrenal medullary cells.
Stachowiak MK, Moffett J, Joy A, Puchacz E, Florkiewicz R, Stachowiak EK.
J Cell Biol. 1994 Oct; 127(1):203-23.
[J Cell Biol. 1994]
- » See all...